Genotyping and detection of common avian and human origin-influenza viruses using a portable chemiluminescence imaging microarray

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Table 4 The limit of detection of the microarray

Virus	Dilution	Micorarray results	Real time PCR results (Ct)	Virus	Dilution	Micorarray results	Real time PCR results (Ct)
H7N9	Original	H7 + N9 + A	23.7	H1N1	Original	H1N1 + A	14.97
	10⁻¹	H7 + N9 + A	28.22		10⁻¹	H1N1 + A	17.49
	10⁻²	H7 + N9 + A	31.79		10⁻²	H1N1 + A	21.55
	10⁻³	–	36.69		10⁻³	H1N1 + A	26.21
	10⁻⁴	–	–		10⁻⁴	H1N1 + A	30.78
	10⁻⁵	–	–		10⁻⁵	A	33.79
H5N1	Original	H5N1 + A	22.55	H3N2	Original	H3N2 + A	15.7
	10⁻¹	H5N1 + A	27.98		10⁻¹	H3N2 + A	18.68
	10⁻²	–	35.99		10⁻²	H3N2 + A	22.72
	10⁻³	–	–		10⁻³	H3N2 + A	27.08
	10⁻⁴	–	–		10⁻⁴	H3N2 + A	31.01
	10⁻⁵	–	–		10⁻⁵	–	33.29
PH1N1	Original	PH1N1 + A	16.24	B	Original	B	18.41
	10⁻¹	PH1N1 + A	19.41		10⁻¹	B	22.53
	10⁻²	PH1N1 + A	23.3		10⁻²	B	26.92
	10⁻³	PH1N1 + A	27.52		10⁻³	B	31.01
	10⁻⁴	PH1N1 + A	32.72		10⁻⁴	–	36.56
	10⁻⁵	–	–		10⁻⁵	–	–

The real-time PCR reagents for influenza A and B viruses were influenza A virus and B virus Nucleic Acid Detection Kits (PCR-fluorescent probe) (Shenzhen Puruikang Biotech Co., Ltd.). The microarray possessed similar or tenfold higher LOD than the real-time RT-PCR method. The reference PFU given for the PCR kits are approximated 1 × 10³ PFU/ml given by the manufacturers and vary between the kits