Skip to main content

Advertisement

Table 4 The limit of detection of the microarray

From: Genotyping and detection of common avian and human origin-influenza viruses using a portable chemiluminescence imaging microarray

Virus Dilution Micorarray results Real time PCR results (Ct) Virus Dilution Micorarray results Real time PCR results (Ct)
H7N9 Original H7 + N9 + A 23.7 H1N1 Original H1N1 + A 14.97
  10−1 H7 + N9 + A 28.22   10−1 H1N1 + A 17.49
  10−2 H7 + N9 + A 31.79   10−2 H1N1 + A 21.55
  10−3 36.69   10−3 H1N1 + A 26.21
  10−4   10−4 H1N1 + A 30.78
  10−5   10−5 A 33.79
H5N1 Original H5N1 + A 22.55 H3N2 Original H3N2 + A 15.7
  10−1 H5N1 + A 27.98   10−1 H3N2 + A 18.68
  10−2 35.99   10−2 H3N2 + A 22.72
  10−3   10−3 H3N2 + A 27.08
  10−4   10−4 H3N2 + A 31.01
  10−5   10−5 33.29
PH1N1 Original PH1N1 + A 16.24 B Original B 18.41
  10−1 PH1N1 + A 19.41   10−1 B 22.53
  10−2 PH1N1 + A 23.3   10−2 B 26.92
  10−3 PH1N1 + A 27.52   10−3 B 31.01
  10−4 PH1N1 + A 32.72   10−4 36.56
  10−5   10−5
  1. The real-time PCR reagents for influenza A and B viruses were influenza A virus and B virus Nucleic Acid Detection Kits (PCR-fluorescent probe) (Shenzhen Puruikang Biotech Co., Ltd.). The microarray possessed similar or tenfold higher LOD than the real-time RT-PCR method. The reference PFU given for the PCR kits are approximated 1 × 103 PFU/ml given by the manufacturers and vary between the kits