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Table 3 Cytotoxicity of the tested extracts and doxorubicin towards sensitive and drug-resistant cancer cell lines and normal cells as determined by the resazurin assay

From: Cytotoxicity of methanol extracts of Annona muricata, Passiflora edulis and nine other Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines

Cell lines

Samples, IC50 values in µg/mL and degrees of resistancea (in bracket)

Doxorubicin

AFB

AML

AMS

AMP

EPW

PSL

PSB

PSR

PEF

PEP

CEM/ADR5000

29.49 ± 1.77 (1.56)

5.25 ± 0.38 (9.29)

6.65 ± 0.22 (18.47)

23.70 ± 1.64 (5.17)

37.00 ± 2.17 (1.99)

18.21 ± 1.45 (1.34)

14.97 ± 0.97 (0.87)

10.13 ± 0.88 (0.59)

8.20 ± 1.02 (11.88)

18.40 ± 1.42 (5.40)

108.00 ± 7.92 (975.60)

MDA-MB-231-pcDNA

>80

>80

>80

>80

>80

52.08 ± 4.98

52.66 ± 6.03

37.19 ± 2.74

>80

>80

0.61 ± 0.15

MDA-MB-231-BCRP

Degree of resistance

>80

>80

>80

>80

>80

61.98 ± 4.31 (1.19)

47.27 ± 3.76 (0.90)

46.92 ± 4.89 (1.26)

>80

>80

4.33 ± 0.26 (7.12)

HCT116 (p53 +/+)

>80

>80

>80

>80

>80

65.21 ± 7.15

34.35 ± 1.99

28.66 ± 1.62

>80

>80

0.78 ± 0.16

HCT116 (p53 /)

Degree of resistance

>80

>80

>80

>80

>80

56.97 ± 4.09 (0.87)

65.68 ± 4.80 (1.91)

72.01 ± 5.26 (2.51)

>80

>80

2.25 ± 0.04 (2.88)

U87MG

>80

>80

>80

>80

>80

65.21 ± 5.79

52.46 ± 5.22

24.80 ± 1.36

>80

>80

0.59 ± 0.08

U87MG.ΔEGFR

Degree of resistance

>80

>80

>80

>80

>80

68.65 ± 3.48 (1.05)

58.70 ± 3.67 (1.12)

46.91 ± 3.01 (1.89)

>80

>80

3.38 ± 0.32 (5.76)

HepG2

38.69 ± 3.08 (>802.07)

>80

>80

>80

>80

46.98 ± 3.17 (>1.70)

36.39 ± 3.08 (0.62)

37.56 ± 2.17 (0.80)

>80

>80

2.12 ± 0.52 (>37.74)

AML12

Degree of resistance

>80

>80

>80

>80

>80

>80

>80

>80

>80

>80

>80

  1. aThe degree of resistance was determined as the ratio of IC50 value in the resistant divided by the IC50 in the sensitive cell line; CEM/ADR5000, MDA-MB-231-BCRP, HCT116 (p53 /), U87MG.ΔEGFR and AML12 were used as the corresponding resistant counterpart for CCRF–CEM (Table 1), MDA-MB-231-pcDNA, HCT116 (p53 +/+), U87MG and HepG2 respectively; the tested methanol extracts were from AFB: Alchornea floribunda bark; AML: Annona muricata leaves; AMS: Annona muricata seeds; AMP: Annona muricata fruit pericarp; EPW: Euphorbia prostata whole plant; PSL: Pachypodanthium staudtii leaves; PSB: Pachypodanthium staudtii bark; PSR: Pachypodanthium staudtii roots; PEF: Passiflora edulis fruit; PEP: Passiflora edulis fruit pericarp; in italics: significant activity