Skip to main content

Table 3 Cytotoxicity of the tested extracts and doxorubicin towards sensitive and drug-resistant cancer cell lines and normal cells as determined by the resazurin assay

From: Cytotoxicity of methanol extracts of Annona muricata, Passiflora edulis and nine other Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines

Cell lines Samples, IC50 values in µg/mL and degrees of resistancea (in bracket) Doxorubicin
AFB AML AMS AMP EPW PSL PSB PSR PEF PEP
CEM/ADR5000 29.49 ± 1.77 (1.56) 5.25 ± 0.38 (9.29) 6.65 ± 0.22 (18.47) 23.70 ± 1.64 (5.17) 37.00 ± 2.17 (1.99) 18.21 ± 1.45 (1.34) 14.97 ± 0.97 (0.87) 10.13 ± 0.88 (0.59) 8.20 ± 1.02 (11.88) 18.40 ± 1.42 (5.40) 108.00 ± 7.92 (975.60)
MDA-MB-231-pcDNA >80 >80 >80 >80 >80 52.08 ± 4.98 52.66 ± 6.03 37.19 ± 2.74 >80 >80 0.61 ± 0.15
MDA-MB-231-BCRP
Degree of resistance
>80 >80 >80 >80 >80 61.98 ± 4.31 (1.19) 47.27 ± 3.76 (0.90) 46.92 ± 4.89 (1.26) >80 >80 4.33 ± 0.26 (7.12)
HCT116 (p53 +/+) >80 >80 >80 >80 >80 65.21 ± 7.15 34.35 ± 1.99 28.66 ± 1.62 >80 >80 0.78 ± 0.16
HCT116 (p53 /)
Degree of resistance
>80 >80 >80 >80 >80 56.97 ± 4.09 (0.87) 65.68 ± 4.80 (1.91) 72.01 ± 5.26 (2.51) >80 >80 2.25 ± 0.04 (2.88)
U87MG >80 >80 >80 >80 >80 65.21 ± 5.79 52.46 ± 5.22 24.80 ± 1.36 >80 >80 0.59 ± 0.08
U87MG.ΔEGFR
Degree of resistance
>80 >80 >80 >80 >80 68.65 ± 3.48 (1.05) 58.70 ± 3.67 (1.12) 46.91 ± 3.01 (1.89) >80 >80 3.38 ± 0.32 (5.76)
HepG2 38.69 ± 3.08 (>802.07) >80 >80 >80 >80 46.98 ± 3.17 (>1.70) 36.39 ± 3.08 (0.62) 37.56 ± 2.17 (0.80) >80 >80 2.12 ± 0.52 (>37.74)
AML12
Degree of resistance
>80 >80 >80 >80 >80 >80 >80 >80 >80 >80 >80
  1. aThe degree of resistance was determined as the ratio of IC50 value in the resistant divided by the IC50 in the sensitive cell line; CEM/ADR5000, MDA-MB-231-BCRP, HCT116 (p53 /), U87MG.ΔEGFR and AML12 were used as the corresponding resistant counterpart for CCRF–CEM (Table 1), MDA-MB-231-pcDNA, HCT116 (p53 +/+), U87MG and HepG2 respectively; the tested methanol extracts were from AFB: Alchornea floribunda bark; AML: Annona muricata leaves; AMS: Annona muricata seeds; AMP: Annona muricata fruit pericarp; EPW: Euphorbia prostata whole plant; PSL: Pachypodanthium staudtii leaves; PSB: Pachypodanthium staudtii bark; PSR: Pachypodanthium staudtii roots; PEF: Passiflora edulis fruit; PEP: Passiflora edulis fruit pericarp; in italics: significant activity