From: Statistical optimization for enhanced yields of probiotic Bacillus coagulans and its phage resistant mutants followed by kinetic modelling of the process
Variables
Optimized shake flask conditions for probiotic strains
B. coagulans
Mutant B. co PIII
Mutant B. co MIII
Glucose (g/l)
59
47
32.5
C/N ratio
42
36
31
Agitation (rpm)
225
250
220
Biomassa (g/l)
7.88
6.3
6.1