Table 5 EVOP designing for the three probiotic cultures and effects of error limits
Parameters | E18 (−1) | E19 (0) | E20 (+1) | E21 (−1) | E22 (0) | E23 (+1) | E24 (−1) | E25 (0) | E26 (+1) |
|---|---|---|---|---|---|---|---|---|---|
(a) B. coagulans (parental strain) | |||||||||
Glucose (g/l) | 53 | 55.8 | 59 | 57 | 59 | 60 | 58 | 59 | 59.5 |
C/N ratio | 38 | 40 | 42 | 41 | 42 | 43 | 41.5 | 42 | 42.5 |
Agitation (rpm) | 200 | 225 | 250 | 240 | 250 | 250 | 245 | 250 | 250 |
Responsea (biomass—g/l) | 7.2 | 7.38 | 7.88 | 7.2 | 7.6 | 7.42 | 7.3 | 7.5 | 7.2 |
Parameters | E18 (−1) | E19 (0) | E20 (+1) | E21 (−1) | E22 (0) | E23 (+1) |
|---|---|---|---|---|---|---|
(b) Mutant B.co PIII | ||||||
Glucose (g/l) | 38 | 44 | 50 | 47 | 50 | 53 |
C/N ratio | 33 | 35 | 37 | 36 | 37 | 38 |
Agitation (rpm) | 200 | 225 | 250 | 230 | 240 | 250 |
Responsea (biomass—g/l) | 4.8 | 5.6 | 6.1 | 6.3 | 6.2 | 5.8 |
Parameters | E18 (−1) | E19 (0) | E20 (+1) | E21 (−1) | E22 (0) | E23 (+1) |
|---|---|---|---|---|---|---|
(c) Mutant B.co MIII | ||||||
Glucose (g/l) | 22.5 | 30 | 37.5 | 32.5 | 37.5 | 42.5 |
C/N ratio | 28 | 30 | 32 | 31 | 32 | 33 |
Agitation (rpm) | 175 | 200 | 225 | 220 | 230 | 240 |
Responsea (biomass—g/l) | 4.82 | 5.2 | 5.56 | 6.1 | 6 | 5.8 |
Error limits | Formulae | B. coagulans | B.co PIII | B.co MIII |
|---|---|---|---|---|
(d) Summary of effects of error limit values of EVOP cycles | ||||
Averages | ±1.414 × σ | 0.577 | 0.409 | 0.363 |
Effects | ±1.004 × σ | 0.954 | 0.677 | 0.601 |
Change in mean | ±0.891 × σ | 0.814 | 0.578 | 0.513 |