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Table 2 Calculation worksheet for analysis of effects, standard deviation and error limits for higher productivity

From: Statistical optimization for enhanced yields of probiotic Bacillus coagulans and its phage resistant mutants followed by kinetic modelling of the process

Effect of variables

Formulae to calculate effects of variables

Glucose

1/6 [X3 + X4 + X5 + X7 + X8 + X9] − [X1 + X2 + X6 + X10 + X11 + X12]

C/N

1/6 [X1 + X3 + X5 + X9 + X11 + X12] − [X2 + X4 + X6 + X7 + X8 + X10]

Agitation

1/6 [X1 + X2 + X7 + X8 + X9 + X11] − [X3 + X4 + X5 + X6 + X10 + X12]

Minerals conc.

1/6 [X4 + X6 + X7 + X9 + X11 + X12] − [X1 + X2 + X3 + X5 + X8 + X10]

  1. X = response, C/N = carbon–nitrogen ratio
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