Table 1 Plackett–Burman design for shake flask optimization
Runs | Variables | Response (biomass—g/l) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | B | C | D | E | F | G | H | B. coagulans | Mutant B. co PIII | Mutant B. co MIII | ||||
Act. | Pred. | Act. | Pred. | Act. | Pred. | |||||||||
1 | −1 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | 3.58 | 3.585 | 3.89 | 4.293 | 2.89 | 2.843 |
2 | −1 | −1 | +1 | −1 | +1 | −1 | −1 | +1 | 3.57 | 3.862 | 3.03 | 3.535 | 2.21 | 2.243 |
3 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | −1 | 2.95 | 3.198 | 3.89 | 4.183 | 2.52 | 2.400 |
4 | +1 | −1 | −1 | −1 | +1 | +1 | +1 | +1 | 2.75 | 2.720 | 2.61 | 3.425 | 2.07 | 2.297 |
5 | +1 | +1 | −1 | −1 | −1 | −1 | −1 | +1 | 4.52 | 4.542 | 4.12 | 4.183 | 2.75 | 2.837 |
6 | −1 | −1 | −1 | −1 | −1 | +1 | −1 | −1 | 2.37 | 2.13 | 2.41 | 2.303 | 2.37 | 2.157 |
7 | +1 | −1 | +1 | +1 | +1 | +1 | −1 | −1 | 5.35 | 5.043 | 5.1 | 4.657 | 3.41 | 3.317 |
8 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | 4.8 | 4.877 | 4.91 | 4.657 | 3.23 | 3.257 |
9 | +1 | +1 | +1 | −1 | −1 | +1 | +1 | −1 | 6.12 | 6.110 | 5.89 | 5.415 | 4.48 | 4.353 |
10 | −1 | −1 | −1 | +1 | −1 | −1 | +1 | −1 | 2.67 | 2.882 | 2.82 | 2.303 | 1.64 | 1.660 |
11 | −1 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | 4.23 | 4.173 | 4.03 | 4.293 | 3.57 | 3.777 |
12 | −1 | +1 | −1 | +1 | +1 | +1 | +1 | +1 | 2.98 | 2.772 | 3.61 | 3.062 | 2.32 | 2.320 |