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Table 5 Effects of dietary PCA on intestinal antioxidant and immune status of LPS-induced oxidative stress broilers fed 1 to 21 days age

From: The protective effect of procyanidin against LPS-induced acute gut injury by the regulations of oxidative state

Items

LPS (−)

LPS (+)

P values

Control

PCA1

PCA2

PCA3

Control

PCA1

PCA2

PCA3

Diet

Stress

Interaction

Jejunum

SOD (U/mg pro)

48.01 ± 2.01b

48.84 ± 2.12b

49.53 ± 2.03b

56.12 ± 1.84c

41.73 ± 1.92a

45.99 ± 1.97a

46.87 ± 1.94a

47.03 ± 1.98b

0.001

0.001

0.054

GSH-Px (U/mg pro)

12.65 ± 1.57b

13.94 ± 1.24b

15.27 ± 1.42b

22.14 ± 1.15c

9.13 ± 1.20a

9.25 ± 1.04a

9.28 ± 0.98a

9.34 ± 0.87a

0.001

0.001

0.001

MDA (nmol/mg pro)

0.63 ± 0.12a

0.61 ± 0.14a

0.58 ± 0.09a

0.51 ± 0.14a

1.98 ± 0.17c

1.21 ± 0.20b

1.16 ± 0.21b

1.02 ± 0.14b

0.001

0.001

0.088

Ileum

SOD (U/mg pro)

40.34 ± 1.76b

41.62 ± 2.13b

42.18 ± 1.82b

42.24 ± 2.07b

37.12 ± 1.95a

39.45 ± 2.34a

40.20 ± 1.64b

40.12 ± 2.21b

0.001

0.197

0.076

GSH-Px (U/mg pro)

10.77 ± 2.14a

11.26 ± 3.01a

17.13 ± 2.88b

19.94 ± 1.27b

10.21 ± 2.07a

10.57 ± 1.47a

11.85 ± 1.55a

12.21 ± 2.01a

0.001

0.061

0.054

MDA (nmol/mg pro)

0.37 ± 0.12a

0.32 ± 0.17a

0.30 ± 0.08a

0.29 ± 0.11a

0.46 ± 0.13b

0.39 ± 0.17a

0.34 ± 0.09a

0.30 ± 0.14a

0.001

0.001

0.051

  1. Data represent means from 6 replicates per treatment
  2. The P values represent the main effect of the diet, the main effect of LPS challenge and the interaction between the dietary treatments and LPS challenge
  3. a, b Least-square mean values (n 6) within a row with unlike superscript letters were significantly different (P < 0.05)
  4. LPS (−) Dietary treatment were abdominal injections sterile saline (0.5 mg/kg body weigh); LPS (+) Dietary treatment were abdominal injections LPS (0.5 mg/kg body weigh); Control Basal diet; PCA1 Basal diet supplemented with 0.05 % PCA; PCA2 Basal diet supplemented with 0.075 % PCA; PCA3 Basal diet supplemented with 0.1 % PCA