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Table 3 Genes involved in co-resistance of Cr(VI) and ampicillin were tested by RNA-seq and qPCR methods in strain LZ-01

From: Multidrug resistance operon emrAB contributes for chromate and ampicillin co-resistance in a Staphylococcus strain isolated from refinery polluted river bank

Genes

Gene annotation

RNA-seq

(0.4 mM Cr(VI))

Fold change

qPCR

(0.2 mg ml−1 Amp)

Fold change

Multidrug transporter and resistance associated genes

SAV0198

ABC-type antimicrobial peptide transport system

6.21

0.61

SAV0199

ABC-type antimicrobial peptide transport system

10.14

0.72

SAV0203

ABC-type multidrug transport system

7.92

0.86

SAV0274

Drug transporter

5.78

0.57

SAV0275

Penicillin amidase V

2.96

0.72

SAV0276

Peptidoglycan hydrolase

1.58

0.47

SAV0277

ABC-type multidrug transport system

1.14

0.79

SAV0351

ABC-type multidrug transport system

1.07

1.18

SAV0661

ABC-type antimicrobial peptide transport system

1.86

1.19

SAV1035

ABC-type antimicrobial peptide transport system

8.23

0.30

SAV1318

ABC-type multidrug transport system

5.10

0.97

SAV1837

ABC-type multidrug transport system

1.01

0.99

SAV1866

ABC-type multidrug transport system

1.23

0.71

SAV2166

Drug resistance MFS transporter

1.47

0.58

SAV2168

Putative multidrug transporter

4.26

1.05

SAV2169

Multidrug ABC transporter

3.58

1.01

SAV2261

Cation/multidrug efflux pump

1.25

1.13

SAV2262

Uncharacterized protein involved in methicillin resistance

1.01

1.12

SAV2265

Mar R family transcriptional regulator

10.64

0.67

SAV2266

MFS family transporter ybf D

6.44

0.63

SAV2352(emrB)

Drug resistance MFS transporter, drug:H+ antiporter-2 family

2.14

2.34a

SAV2353(emrA)

Multidrug resistance efflux pump

1.29

1.60a

SAV2355

Drug resistance transporter, Bcr/Cfl A subfamily

3.60

0.86

SAV2419

Toxin production and resistance

1.28

0.94

SAV2420

Toxin production and resistance

1.22

0.94

SAV2421

Toxin production and resistance

1.51

0.59

SAV2428

ABC-type multidrug transport system

15.80

0.51

SAV2462

Antibiotic resistance protein

2.13

0.79

SAV2552

Predicted drug exports of the RND family

4.55

0.66

SAV2623

ABC-type antimicrobial peptide transport system

1.68

0.96

SAV2701

ABC-type antimicrobial peptide transport system

13.05

0.73

SAV2702

Similar to ABC transporter, vraE protein

10.66

0.63

Ampicillin-resistant genes

SAV1275

Metallo-beta-lactamase superfamily

0.98

0.80

SAV1504

Metallo-beta-lactamase

1.02

0.51

SAV1545

Metallo-beta-lactamase superfamily protein

0.74

1.02

SAV2441

Similar to beta-lactamase

1.42

0.44

pbpA

Penicillin-binding protein 1

1.54

3.88

pbp2

Penicillin-binding protein 1A

2.10

2.02

pbp3

Penicillin-binding protein 3

1.05

2.71

pbp4

d-alanyl-d-alanine carboxypeptidase (penicillin-binding protein)

0.77

3.32

  1. aStands for the selected genes for next study