Table 2 Details of ISSR primers used to analyze genetic diversity in 30 cardamom accessions
Sl. no. | ISSR primer | Sequence | Ta (ËšC) |
|---|---|---|---|
1 | S807 | 5′-TATATATATATATATAC-3′ | 50.0 |
2 | S809 | 5′-AGAGAGAGAGAGAGAGC-3′ | 52.0 |
3 | S811 | 5′-GAGAGAGAGAGAGAGAT-3′ | 50.4 |
4 | S813 | 5′-GAGAGAGAGAGAGAGAC-3′ | 52.0 |
5 | S817 | 5′-CACACACACACACACAT-3′ | 50.0 |
6 | S820 | 5′-CACACACACACACACAA-3′ | 50.0 |
7 | S824 | 5′-TCTCTCTCTCTCTCTCC-3′ | 52.0 |
8 | S826 | 5′-TCTCTCTCTCTCTCTCG-3′ | 52.0 |
9 | S829 | 5′-ACACACACACACACC-3′ | 52.0 |
10 | S834 | 5′-TGTGTGTGTGTGTGC-3′ | 52.0 |
11 | S835 | 5′-AGAGAGAGAGAGAGAGYT-3′ | 50.0 |
12 | S836 | 5′-AGAGAGAGAGAGAGAGYC-3′ | 50.0 |
13 | S842 | 5′-GAGAGAGAGAGAGAGAYC-3′ | 53.7 |
14 | S844 | 5′-CTCTCTCTCTCTCTCTRA-3′ | 50.0 |
15 | S845 | 5′-CTCTCTCTCTCTCTCTRC-3′ | 53.0 |
16 | S849 | 5′-CACACACACACACACARG-3′ | 53.7 |
17 | S850 | 5′-GTGTGTGTGTGTGTGTCA-3′ | 53.7 |
18 | S851 | 5′-GTGTGTGTGTGTGTGTTC-3′ | 55.8 |
19 | S855 | 5′-TCTCTCTCTCTCTCTCRG-3′ | 51.0 |
20 | S857 | 5′-ACACACACACACACACY-3′ | 53.0 |
21 | S860 | 5′-ACACACACACACACACTG-3′ | 53.0 |
22 | S864 | 5′-TGTGTGTGTGTGTGA-3′ | 47.0 |
23 | S868 | 5′-CTCCTCCTCCTCCTCCTC-3′ | 47.0 |
24 | S872 | 5′-GAAGAAGAAGAAGAAGA-3′ | 40.0 |
25 | S880 | 5′-CTTCACTTCACTTCA-3′ | 51.0 |
26 | S895 | 5′-GGAGAGGAGAGGAGA-3′ | 51.0 |