Assessment of mangroves from Goa, west coast India using DNA barcode

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Table 3 Identification success rates using TaxonDNA (Species Identifier) program under ‘Best Match’ and ‘Best Closest Match’ methods

Barcodes	No. of Sequences	Best Match (%)			Best closest match (%)				T (%)	No. of clusters	Match/mismatch
Barcodes	No. of Sequences	Correct	Ambiguous	Incorrect	Correct	Ambiguous	Incorrect	No match	T (%)	No. of clusters	Match/mismatch
rbcL	44	47.72	36.36	15.9	31.81	27.27	11.36	13	0	23	6/8
matK	43	72.09	25.58	2.32	39.53	13.95	2.32	44.18	0.11	24	10/4
rbcL + matK	42	66.66	16.66	16.66	66.66	16.66	16.66	0	0.2	21	8/6

TaxonDNA is an alignment-based method based on sequence distance matrices. Percentage of correct/incorrect/ambiguous assignment of a taxon is compared using molecular operating taxonomic unit (MOTU). The species specific clustering using match and mismatch criteria
T Threshold