Fig. 2

Niclosamide inhibits Wnt/β-catenin signaling in RCC cells. a Niclosamide decreases intracellular β-catenin levels in A-498 and SW-839 cells. Cells were treated with niclosamide for 24 h prior to WB analysis. b Niclosamide inhibits TOPflash activation. RCC cells transfected with TOPflash plasmid were treated with niclosamide as indicated. RLU, relative light units. c β-Catenin level is increased n A-498 and SW-839 cells transfected with β-catenin overexpression plasmid. Overexpression of β-catenin partially rescued the effects of niclosamide in inhibiting proliferation (d) and colony formation (e) and inducing apoptosis (f) in RCC cells. *p < 0.05, compared to control or p-Vector