Table 1 Sequences of primers used to identify HCoVs in this study
From: Molecular epidemiology and characterization of human coronavirus in Thailand, 2012–2013
Gene | Primer | Genotype | Sequence (5′–3′)a | PCR application |
|---|---|---|---|---|
RdRp | SP6-CoV_16053_F | ATTTAGGTGACACTATAGGGTTGGGAY TAYCCTAARTGTGA | First and second round | |
CoV-16594_R | TAYTATCARAAYAATGTCTTTATGTC | First round | ||
CoV-Pan_16510_R | TGATGATGGNGTTGTBTGYTATAA | Second round | ||
S | HCoV-S229E_F1 | 229E | GTGGGTGCACTACCTAAGAC | First round |
HCoV-S229E_R1 | 229E | CGTGGTTGAACAGCAATTATAGAACC | ||
HCoV-S229E_F2 | 229E | GAGTTTGTTATTTCACGCACAGGAC | Second round | |
HCoV-S229E_R2 | 229E | CCATCTGCACAAACGCCAAAAC | ||
HCoV-SHKU1_F1 | HKU1 | TCACCTCTTAATTGGGAACGTA | First round | |
HCoV-SHKU1_R1 | HKU1 | CATTAGAACAAGTGGTGCCAC | ||
HCoV-SHKU1_F2 | HKU1 | GATTTGCAGTTGGGCAGTTCTGG | Second round | |
HCoV-SHKU1_R2 | HKU1 | AAAGGCATCAGGACTACAAA | ||
HCoV-SNL63_F1 | NL63 | GACACCACAATACCTTTTGG | First round | |
HCoV-SNL63_R1 | NL63 | CTGGTTGGTTACATGGTGTCAC | ||
HCoV-SNL63_F2 | NL63 | CATGTTAGCACTTTTGTGGGT | Second round | |
HCoV-SNL63_R2 | NL63 | CCACCAGCAAGTGACTGGTTTG | ||
HCoV-SOC43_F1 | OC43 | GTCGGTGCCCTCTCCATTAAATT | First round | |
HCoV-SOC43_R1 | OC43 | GGCCGCAGAAACACGAC | ||
HCoV-SOC43_F2 | OC43 | AATATGAGCAGCCTGATGTC | Second round | |
HCoV-SOC43_R2 | OC43 | CCGAAATAGCAATGCTGGTTC | ||
N | NSeq-Fwd | MERS | CCTTCGGTACAGTGGAGCCA | First round |
NSeq-Fnest | MERS | TGACCCAAAGAATCCCAACTAC | Second round | |
NSeq-Rev | MERS | GATGGGGTTGCCAAACACAAAC | First and second round |