Fig. 1
From: Detection of mesenchymal stem cells senescence by prelamin A accumulation at the nuclear level
MSC undergo replicative senescence during in vitro expansion. a Cumulative population doublings (CPD) of cell cultures from three different donors. b Passages and PDT intervals corresponding to the early and late stages of each MSC cell line. The end of the late stage matches with replicative senescence of the culture. c Representative brightfield images of MSC at early and late stages. White boxes indicate areas of magnification highlighting the altered shape of senescent cells compared to the well defined spindle-shape of MSC at early stages. Scale bar 200 µm. d Doubling times (DT) calculated from proliferation assay confirm the remarked reduction of proliferation potential of cells according to the progression of life-span stages