Biochemical effect of a histidine phosphatase acid (phytase) of Aspergillus japonicus var. Saito on performance and bony characteristics of broiler

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Table 2 Composition of the experimental diets for broilers from 22 to 42 days old

	Calculated values
Metabolizable energy (kcal/kg)	2.950	2.953	2.953	2.955	2.955
Crude protein	195.0	195.0	195.0	195.0	195.0
Calcium	8.300	8.300	8.300	8.300	8.300
Choline chloride	0.00	0.00	0.00	0.00	0.00
Available phosphorus	3.967	3.967	3.967	3.967	3.967
Total phosphorus	6.500	6.132	6.132	5.764	5.764
Sodium	2.200	2.200	2.200	2.200	2.200
Digestible lysine	10.700	10.700	10.700	10.700	10.700
Digestible methionine	4.992	4.992	4.992	4.991	4.991
Digestible methionin + cystine	7.700	7.700	7.700	7.700	7.700
Digestible threonine	6.574	6.574	6.574	6.574	6.574
Digestible tryptophan	2.187	2.186	2.186	2.186	2.186
Digestible valine	8.058	8.058	8.058	8.057	8.057
Digestible arginine	11.943	19.41	19.41	11.939	11.939
Digestible isoleucine	7.544	7.543	7.543	7.542	7.542

Enrichment per kilogram of diet: retinol, 25 mg; cholecalciferol, 0.38 mg; dl-tocopheryl acetate, 20 mg; phylloquinone, 0.5 mg; thiamin, 2 mg; riboflavin, 3.6 mg; cyanocobalamin, 20 µg; calcium pantothenate, 8 mg; folic acid, 0.3 mg; growth promoter, 50 mg; niacin, 30 mg; Cu, 40 mg; I, 1.25 mg; Se, 0.25 mg; Mn, 80 mg; Zn, 80 mg; Fe, 35 mg; antioxidant, 0.5 mg; coccidiostat, 110 mg
^aT1—control diet without enzyme and with available phosphorus according Tables for Broilers (Rostagno 2005); T2—250 FTU/kg of feed and 33 % more phytic phosphorus than T1; T3—500 FTU/kg of feed and 33 % more phytic phosphorus than T1; T4—250 FTU/kg of feed and 66 % more phytic phosphorus than T1; T5—500 FTU/kg of feed and 66 % more phytic phosphorus than T1; FTU—phytasic unit