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Fig. 5 | SpringerPlus

Fig. 5

From: A synthetic biology standard for Chinese Hamster Ovary cell genome monitoring and contaminant detection by polymerase chain reaction

Fig. 5

Qualitative comparison of SC qPCR and LRE qPCR for quantitation of a genomic sequence. The same samples as used in Fig. 4 were used to assess LRE qPCR (open triangles) and SC qPCR (open circles) performance. Copies of the GAPDH target sequence present in a sample were measured by each method and plotted as a function of sample dilution for samples derived from shake flask (a) and bioreactor (b) cultivation. Grey circles indicate genome copy number inferred from a spectrophotometric measurement of total DNA concentration present in sample. The dashed lines indicate linear extrapolation of the spectrophotometric data points

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