Fig. 1

Gel analysis of gDNA and overview of polymerase chain reactions in this study. Primers (black triangles) detailed in Table 2 were used to amplify a mycoplasma sequence present in the plasmid pPROX2 (3010 bp) as a proxy for pathogen detection (a), target DNA within the mammalian GAPDH gene in the CHO genome (b) and the designated CAL1 locus with the lambda phage genome (c). Expected amplicon size (bp) is indicated under the bar at the bottom of each panel. (d) 60 µL of a 2.5 × 10⁶ cell/mL cell suspension was ran on a gel before sonication in the lane marked ‘C’. 60 µL of a 2.5 × 10⁶ cell/mL cell suspension was ran on a gel after sonication in the lane marked ‘S’. Molecular weight ladder was run in leftmost lane, uppermost band is 10 kilo-base pairs