CRISPR-Cas9 directed knock-out of a constitutively expressed gene using lance array nanoinjection

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Table 1 LAN Statistical summary of the sample types and associated GFP KO rates

Sample type	Sample size (n)	Mean GFP KO percent (%)	Median GFP KO percent (%)
NTC	21	5.27	5.37
NC	26	3.92	3.62
BC	18	5.96	5.37
DC	23	4.04	3.82
1.5 mA, x1	16	6.92	6.11
4.5 mA, x1	8	21.63	17.37
6.0 mA, x1	16	22.65	8.45
1.5 mA, x3	20	66.79	72.78
4.5 mA, x3	27	79.56	93.77
6.0 mA, x3	20	70.01	70.32

Data collected from flow cytometry and analyzed in JMP represents mean and median GFP KO rates for the respective sample types. Percentage of cells successfully transfected is calculated as the number of living and GFP negative cells divided by the number of living cells in each sample