Skip to main content

Table 3 Results from respiratory chain activity when different substrates obtained from different mitochondrial preparations for each experimental group energized with pyruvate-malate or succinate (5 mM)

From: In vivo study of hepatic oxidative stress and mitochondrial function in rabbits with severe hypotension after propofol prolonged infusion

Parameter Group Statistical test p
Saline SMOFlipid Propofol Lipuro
Pyruvate-malate substrate
 State 3 rate (nmol O2 min−1 mg protein−1) 54.66 ± 16.97 47.81 ± 18.04 41.07 ± 15.04 F(2,17) = 1.67 0.34
 State 4 rate (nmol O2 min−1 mg protein−1) 16.82 [13.58–19.60] 20.06 [14.24–28.35] 15.48 [11.23–20.77] χ2(2) = 1.29 0.52
 Uncoupled rate (nmol O2 min−1 mg protein−1) 44.59 ± 17.08 47.99 ± 18.36 50.25 ± 17.99 F(2,17) = 0.18 0.84
 RCR 2.48 [2.20–3.11] 2.34 [1.92–2.91] 2.81 [2.34–3.12] χ2(2) = 3.08 0.21
 ADP/O 2.60 ± [2.34–2.94] 2.85 [2.79–2.91] 2.64 [2.37–2.97] χ2(2) = 0.72 0.69
Succinate substrate
 State 3 rate (nmol O2 min−1 mg protein−1) 51.68 ± 3.42a 38.42 ± 4.15b 45.66 ± 7.29a,b F(2,18) = 9.13 0.002
 State 4 rate (nmol O2 min−1 mg protein−1) 22.99 ± 4.26 20.63 ± 5.83 21.34 ± 3.37 F(2,20) = 2.84 0.09
 Uncoupled rate (nmol O2 min−1 mg protein−1) 177.5 ± 39.4 141.8 ± 35.47 166.8 ± 23.06 F(2,18) = 3.05 0.08
 RCR ou ICR 2.40 ± 0.17 2.06 ± 0.10 2.16 ± 0.12 F(2,18) = 1.59 0.23
 ADP/O 1.34 ± 0.40 1.33 ± 0.39 1.33 ± 0.35 F(2,19) = 0.15 0.86
 Membrane potential 171.7 ± 30.15 145.8 ± 37.5 156.8 ± 26.1 F(2,20) = 1.18 0.33
  1. Results expressed by M ± SD (mean ± standard deviation) or median and interquartile range for liver mitochondria (1 mg mL−1 of protein). Oxidative phosphorylation was measured polarographically at 25 °C in 1 mL. ΔΨ was measured using a TPP+-selective electrode at 25 °C in a total volume of 1 mL. Different letters show significant differences between groups (p < 0.05)