In vivo study of hepatic oxidative stress and mitochondrial function in rabbits with severe hypotension after propofol prolonged infusion

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Table 3 Results from respiratory chain activity when different substrates obtained from different mitochondrial preparations for each experimental group energized with pyruvate-malate or succinate (5 mM)

Parameter	Group			Statistical test	p
Parameter	Saline	SMOFlipid	Propofol Lipuro	Statistical test	p
Pyruvate-malate substrate
State 3 rate (nmol O₂ min⁻¹ mg protein⁻¹)	54.66 ± 16.97	47.81 ± 18.04	41.07 ± 15.04	F(2,17) = 1.67	0.34
State 4 rate (nmol O₂ min⁻¹ mg protein⁻¹)	16.82 [13.58–19.60]	20.06 [14.24–28.35]	15.48 [11.23–20.77]	χ²(2) = 1.29	0.52
Uncoupled rate (nmol O₂ min⁻¹ mg protein⁻¹)	44.59 ± 17.08	47.99 ± 18.36	50.25 ± 17.99	F(2,17) = 0.18	0.84
RCR	2.48 [2.20–3.11]	2.34 [1.92–2.91]	2.81 [2.34–3.12]	χ²(2) = 3.08	0.21
ADP/O	2.60 ± [2.34–2.94]	2.85 [2.79–2.91]	2.64 [2.37–2.97]	χ²(2) = 0.72	0.69
Succinate substrate
State 3 rate (nmol O₂ min⁻¹ mg protein⁻¹)	51.68 ± 3.42^a	38.42 ± 4.15^b	45.66 ± 7.29^a,b	F(2,18) = 9.13	0.002
State 4 rate (nmol O₂ min⁻¹ mg protein⁻¹)	22.99 ± 4.26	20.63 ± 5.83	21.34 ± 3.37	F(2,20) = 2.84	0.09
Uncoupled rate (nmol O₂ min⁻¹ mg protein⁻¹)	177.5 ± 39.4	141.8 ± 35.47	166.8 ± 23.06	F(2,18) = 3.05	0.08
RCR ou ICR	2.40 ± 0.17	2.06 ± 0.10	2.16 ± 0.12	F(2,18) = 1.59	0.23
ADP/O	1.34 ± 0.40	1.33 ± 0.39	1.33 ± 0.35	F(2,19) = 0.15	0.86
Membrane potential	171.7 ± 30.15	145.8 ± 37.5	156.8 ± 26.1	F(2,20) = 1.18	0.33

Results expressed by M ± SD (mean ± standard deviation) or median and interquartile range for liver mitochondria (1 mg mL⁻¹ of protein). Oxidative phosphorylation was measured polarographically at 25 °C in 1 mL. ΔΨ was measured using a TPP⁺-selective electrode at 25 °C in a total volume of 1 mL. Different letters show significant differences between groups (p < 0.05)