Fig. 3

Analysis of intestinal microbial flora of mice fed with koji glycosylceramide. a Cluster tree of microbes increased or decreased in the feces of mice fed with koji glycosylceramide. The underline indicates microbes whose read values were significantly increased (p < 0.05). The broken underline indicates microbes whose percentage values were significantly increased (p < 0.05). The square indicates microbes whose read values and percentage values were significantly increased. Stars indicate microbes which were larger in Kgc than in Nor. b Box plot representing the relative abundance of the genera (Blautia coccoides) enriched in the feces of koji glycosylceramide-fed mice. Mice were fed with koji glycosylceramide for 1 week. The feces were recovered, and freeze-dried. Genomic DNA was extracted and purified from the feces. The V3–V4 variable region of 16S rRNA was amplified using PCR and sequenced using Miseq, Illumina. Data were analyzed by MiSeq Reported 16S metagenomics system. p value indicates one-tailed unpaired Student’s t-test under symmetry conditions (n = 3)