Fig. 2

Metabolic fate of koji glycosylceramide in the intestine. a Total lipid profile of feces from mice fed with or without koji glycosylceramide. Nor indicates the feces of non-added mice and Kgc indicates those of koji glycosylceramide-fed mice. The number indicates the replicate number of experiments. b Total lipid profile of koji glycosylceramide incubated with intestinal extracts. c NBD-TLC of C12-ceramide incubated with intestinal extracts. Intestinal extract was recovered from mice, mixed with purified koji glycosylceramide or NBD-C12-ceramide, incubated at 37 °C for 16–30 h, developed, and visualized by orcinol–H₂SO₄ reagent or fluorescence. SG indicates Sterylglucoside and GlcCer indicates glycosylceramide, a and b indicate hydroxylated and nonhydroxylated cerebroside respectively