From: High quality RNA extraction from Maqui berry for its application in next-generation sequencing
Compound | Function |
---|---|
Hexadecyltrimethyl ammonium bromide (CTAB) | This detergent simultaneously solubilizes the plant cell wall and lipid membranes of internal organelles and denatures proteins (enzymes) |
PVP-40 | Remove phenolic compounds and secondary metabolites from nucleic acid preparations, and it also prevent browning effect of polyphenols |
Spermidine | Binds to and precipitates nucleic acid |
β-Mercapoetanol | An antioxidant/reducing agent that will irreversibly denature RNases by reducing disulfide bonds and destroying the native conformation required for enzyme functionality |
Lithium chloride | Allows the precipitation of RNA because it does not efficiently precipitate either protein or DNA |