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Table 2 Function of the compounds used in the RNA extraction method from Maqui berry

From: High quality RNA extraction from Maqui berry for its application in next-generation sequencing

Compound

Function

Hexadecyltrimethyl ammonium bromide (CTAB)

This detergent simultaneously solubilizes the plant cell wall and lipid membranes of internal organelles and denatures proteins (enzymes)

PVP-40

Remove phenolic compounds and secondary metabolites from nucleic acid preparations, and it also prevent browning effect of polyphenols

Spermidine

Binds to and precipitates nucleic acid

β-Mercapoetanol

An antioxidant/reducing agent that will irreversibly denature RNases by reducing disulfide bonds and destroying the native conformation required for enzyme functionality

Lithium chloride

Allows the precipitation of RNA because it does not efficiently precipitate either protein or DNA