Fig. 3

Intratracheal administration of lipopolysaccharide (LPS) into the lungs of wild-type (WT) and SPC-CCL1 transgenic (Tg) mice. To produce a model of acute lung injury, mice were intratracheally administered LPS (2 mg/kg body weight). a WT mice, 6 h after administration. Inflammatory cells such as neutrophils had infiltrated the alveoli. b SPC-CCL1 Tg mice, 6 h after administration. Infiltration of inflammatory cells was also observed. c WT mice, 72 h after administration. The alveoli were filled with inflammatory cells, particularly neutrophils, macrophages, and exudates. d SPC-CCL1 Tg mice, 72 h after administration. Histopathological findings did not show significant differences between LPS-treated SPC-CCL1 Tg mice and WT mice. e Time course of changes in BAL cells (left total cells, middle macrophages, right neutrophils) 6, 24 and 72 h after LPS administration. CCL1 overexpression changed neither the total number nor population of BAL cells