Fig. 2

Flow cytometric (FCM) plots of BAL cells and alveolar macrophages. Representative schemas for dot plots for FCM are shown. BAL cells of WT mice (a) and SPC-CCL1 Tg mice (b) were distributed by forward scatter (FSC) and side scatter (SSC). Cells regarded as alveolar macrophages were enclosed hexagonal form. Alveolar macrophages were decreased in SPC-CCL1 Tg mice compared to WT mice. FSC and SSC were expressed on a log scale, and smaller objects (FSC < 5000) were omitted from analyses as debris. The fraction of macrophages was gated and the surface markers of WT mice and SPC-CCL1 Tg mice were evaluated. The gated fractions of AMs from WT mice (c) and SPC-CCL1 Tg mice (d) were distributed by FITC-F4/80 and PerCP-CD11b. e F4/80-positive cells were decreased in SPC-CCL1 Tg mice (gray bar) compared to WT mice (black bar). f CD11b-positive cells remained unchanged compared to WT mice. *P value <0.05 compared to WT mice