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Table 1 Column chromatographic separation of chloroform extract

From: Structure elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parviflora

Fractions

Solvent

Volume collected (mL)

Combined fractions

Rf value for major spot

Number of spots

TLC solvent

1–13

PE (100 %)

130

1–13

0.22

6

PE:CHCl3 (4:1)

14–29

PE:CHCl3 (10:1)

160

14–29

0.56

4

PE:CHCl3 (2:1)

30–40

PE:CHCl3 (9:1)

110

34–35

0.42

2

PE:CHCl3 (1:1)

36–40

0.44

3

41–48

PE:CHCl3 (8:1)

80

42–48

0.50

4

PE:CHCl3 (1:2)

49–55

PE:CHCl3 (7:1)

70

49–55

0.50

3

PE:CHCl3 (1:2)

56–62

PE:CHCl3 (6:1)

70

56–62

0.41

2

PE:CHCl3 (1:2)

63–67

PE:CHCl3 (5:1)

50

63–67

0.20

4

CHCl3 (100 %)

68–74

PE:CHCl3 (4:1)

70

68–74

0.20

4

CHCl3 (100 %)

75–89

PE:CHCl 3 (3:1)

150

75–77

0.55

4

CHCl 3 :Act (15:1)

8089

0.50

3

90–101

PE:CHCl3 (2:1)

120

90–101

0.21

3

CHCl3:Act (9:1)

102–133

PE:CHCl3 (1:1)

320

102–110

0.46

2

CHCl3:Act (10:1)

111–118

0.44

3

  1. Italics characters are used for the conditions that the compound has been isolated
  2. PE petroleum ether, CHCl 3 chloroform, Act acetone