Fractions | Solvent | Volume collected (mL) | Combined fractions | Rf value for major spot | Number of spots | TLC solvent |
---|---|---|---|---|---|---|
1–13 | PE (100 %) | 130 | 1–13 | 0.22 | 6 | PE:CHCl3 (4:1) |
14–29 | PE:CHCl3 (10:1) | 160 | 14–29 | 0.56 | 4 | PE:CHCl3 (2:1) |
30–40 | PE:CHCl3 (9:1) | 110 | 34–35 | 0.42 | 2 | PE:CHCl3 (1:1) |
36–40 | 0.44 | 3 | ||||
41–48 | PE:CHCl3 (8:1) | 80 | 42–48 | 0.50 | 4 | PE:CHCl3 (1:2) |
49–55 | PE:CHCl3 (7:1) | 70 | 49–55 | 0.50 | 3 | PE:CHCl3 (1:2) |
56–62 | PE:CHCl3 (6:1) | 70 | 56–62 | 0.41 | 2 | PE:CHCl3 (1:2) |
63–67 | PE:CHCl3 (5:1) | 50 | 63–67 | 0.20 | 4 | CHCl3 (100 %) |
68–74 | PE:CHCl3 (4:1) | 70 | 68–74 | 0.20 | 4 | CHCl3 (100 %) |
75–89 | PE:CHCl 3 (3:1) | 150 | 75–77 | 0.55 | 4 | CHCl 3 :Act (15:1) |
80–89 | 0.50 | 3 | ||||
90–101 | PE:CHCl3 (2:1) | 120 | 90–101 | 0.21 | 3 | CHCl3:Act (9:1) |
102–133 | PE:CHCl3 (1:1) | 320 | 102–110 | 0.46 | 2 | CHCl3:Act (10:1) |
111–118 | 0.44 | 3 |