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Table 1 Column chromatographic separation of chloroform extract

From: Structure elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parviflora

Fractions Solvent Volume collected (mL) Combined fractions Rf value for major spot Number of spots TLC solvent
1–13 PE (100 %) 130 1–13 0.22 6 PE:CHCl3 (4:1)
14–29 PE:CHCl3 (10:1) 160 14–29 0.56 4 PE:CHCl3 (2:1)
30–40 PE:CHCl3 (9:1) 110 34–35 0.42 2 PE:CHCl3 (1:1)
36–40 0.44 3
41–48 PE:CHCl3 (8:1) 80 42–48 0.50 4 PE:CHCl3 (1:2)
49–55 PE:CHCl3 (7:1) 70 49–55 0.50 3 PE:CHCl3 (1:2)
56–62 PE:CHCl3 (6:1) 70 56–62 0.41 2 PE:CHCl3 (1:2)
63–67 PE:CHCl3 (5:1) 50 63–67 0.20 4 CHCl3 (100 %)
68–74 PE:CHCl3 (4:1) 70 68–74 0.20 4 CHCl3 (100 %)
75–89 PE:CHCl 3 (3:1) 150 75–77 0.55 4 CHCl 3 :Act (15:1)
8089 0.50 3
90–101 PE:CHCl3 (2:1) 120 90–101 0.21 3 CHCl3:Act (9:1)
102–133 PE:CHCl3 (1:1) 320 102–110 0.46 2 CHCl3:Act (10:1)
111–118 0.44 3
  1. Italics characters are used for the conditions that the compound has been isolated
  2. PE petroleum ether, CHCl 3 chloroform, Act acetone