From: Genotoxicity reduction in bagasse waste of sugar industry by earthworm technology
Types of chromosomal aberrations | 0 % (control) | 25 % | 50 % | 75 % | 100 % | |||||
---|---|---|---|---|---|---|---|---|---|---|
No. of Aberrant cellsa | No. of Aberrant cellsa | No. of Aberrant cellsa | No. of Aberrant cellsa | No. of Aberrant cellsa | ||||||
6 h | 12 h | 6 h | 12 h | 6 h | 12 h | 6 h | 12 h | 6 h | 12 h | |
Physiological aberrations (PA) | ||||||||||
C-mitosis | 3 (2) | 4 (2) | 7 (4) | 6 (1) | 9 (4) | 8 (5) | 7 (6) | 9 (5) | 9 (9) | 8 (10) |
Delayed anaphase | 4 (4) | 6 (5) | 7 (6) | 12 (10) | 5 (7) | 11 (8) | 13 (10) | 15 (9) | 14 (13) | 19 (14) |
Laggard/s | – | – | – | – | – | – | – | 1 (1) | 2 (1) | 1 (1) |
Stickiness | – | 1 | 3 (1) | 2 (1) | 2 (2) | 3 (2) | 6 (1) | 5 (2) | 6 (2) | 8 (3) |
Vagrant/s | 3 (1) | 1 (1) | 5 (2) | 3 (2) | 6 (2) | 4 (1) | 7 (1) | 6 (2) | 8 (3) | 2 (4) |
Total PA | 10 (7) | 12 (8) | 22(13) | 23 (14) | 22 (15) | 26 (16) | 33 (18) | 36 (19) | 39 (28) | 38 (32) |
Clastogenic aberrations (CA) | ||||||||||
Chromatin bridge/s | – (1) | 3 (2) | 3 (2) | 5 (3) | 5 (3) | 7 (5) | 7 (4) | 7 (5) | 6 (7) | 9 (6) |
Chromosomal break/s | 2 | – | – | 1 | 2 (1) | 2 (1) | 3 (1) | 2 (1) | 2 (1) | 4 (2) |
Total CA | 2 (1) | 3 (2) | 3 (2) | 6 (3) | 7 (4) | 9 (6) | 10 (5) | 9 (6) | 8 (8) | 13 (8) |
Total aberrant cells (PA + CA) | 12 (8) | 15 (10) | 25 (15) | 29 (17) | 29 (19) | 35 (22) | 43 (23) | 45 (25) | 47 (36) | 51 (40) |
Percent aberration (%) | 2.66 (1.77) | 3.33 (2.22) | 5.55 (3.33) | 6.44 (3.77) | 6.44 (4.22) | 7.77 (4.88) | 9.55* (5.11) | 10.0 (5.55) | 10.44* (8.0*) | 11.33* (8.88*) |
Percent reduction (%) | 33.45 | 33.33 | 40.0 | 41.45 | 34.47 | 37.19 | 41.88 | 44.50 | 23.37 | 21.62 |