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Table 2 Enzyme activity assay for KSH and GDH

From: Efficient 9α-hydroxy-4-androstene-3,17-dione production by engineered Bacillus subtilis co-expressing Mycobacterium neoaurum 3-ketosteroid 9α-hydroxylase and B. subtilis glucose 1-dehydrogenase with NADH regeneration

Strains KSH activity
U (mg total protein)−1
GDH activity
U (mg total protein)−1
M. neoaurum JC-12 0.02 ± 0.01
B. subtilis 168 NT 0.03 ± 0.01
B. subtilis 168/pMA5-kshA NT 0.03 ± 0.01
B. subtilis 168/pMA5-ksh 0.57 ± 0.02 0.03 ± 0.01
B. subtilis 168/pMA5-kshgdh 0.53 ± 0.02 0.35 ± 0.02
Purified KshA NT
Co-purified KshA and KshB 2.41 ± 0.05
  1. All assays were performed in triplicate
  2. NT, enzyme activity was not detectable; –, not detect