Table 2 Enzyme activity assay for KSH and GDH
Strains | KSH activity U (mg total protein)−1 | GDH activity U (mg total protein)−1 |
|---|---|---|
M. neoaurum JC-12 | 0.02 ± 0.01 | – |
B. subtilis 168 | NT | 0.03 ± 0.01 |
B. subtilis 168/pMA5-kshA | NT | 0.03 ± 0.01 |
B. subtilis 168/pMA5-ksh | 0.57 ± 0.02 | 0.03 ± 0.01 |
B. subtilis 168/pMA5-ksh–gdh | 0.53 ± 0.02 | 0.35 ± 0.02 |
Purified KshA | NT | – |
Co-purified KshA and KshB | 2.41 ± 0.05 | – |