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Table 1 Primers used in this work

From: Efficient 9α-hydroxy-4-androstene-3,17-dione production by engineered Bacillus subtilis co-expressing Mycobacterium neoaurum 3-ketosteroid 9α-hydroxylase and B. subtilis glucose 1-dehydrogenase with NADH regeneration

Primers Sequences (5′–3′) Restriction sites
KshA-F ATCCATATGACTACCGAGACAGCCG NdeI
KshA-R CGACGCGTTTAGTGGTGGTGGTGGTGGTGGCTCGGCTGCGCGGAC MluI
KshB-F CGCGGATCCATGACGGAGGAGCCGCTC BamHI
KshB-R1 CGCGGATCCCTATTCGTCGTAGGTGACTTC BamHI
HpaII-F1 ACCGGAATTCATGACGGAGGAGCCGCTC EcoRI
KshB-R2 CCCCCCGGGTTAGTGGTGGTGGTGGTGGTGTTCGTCGTAGGTGACTTC SmaI
HpaII-F2 ACCGGGTACCATGACGGAGGAGCCGCTC KpnI
Gdh-R ACCGAAGCTTTTAACCGCGGCCTGCCTGGAATGAAG HindIII
  1. The restriction enzyme sites were bold type