Primers | Sequences (5′–3′) | Restriction sites |
---|---|---|
KshA-F | ATCCATATGACTACCGAGACAGCCG | NdeI |
KshA-R | CGACGCGTTTAGTGGTGGTGGTGGTGGTGGCTCGGCTGCGCGGAC | MluI |
KshB-F | CGCGGATCCATGACGGAGGAGCCGCTC | BamHI |
KshB-R1 | CGCGGATCCCTATTCGTCGTAGGTGACTTC | BamHI |
HpaII-F1 | ACCGGAATTCATGACGGAGGAGCCGCTC | EcoRI |
KshB-R2 | CCCCCCGGGTTAGTGGTGGTGGTGGTGGTGTTCGTCGTAGGTGACTTC | SmaI |
HpaII-F2 | ACCGGGTACCATGACGGAGGAGCCGCTC | KpnI |
Gdh-R | ACCGAAGCTTTTAACCGCGGCCTGCCTGGAATGAAG | HindIII |