Fig. 3From: Efficient 9α-hydroxy-4-androstene-3,17-dione production by engineered Bacillus subtilis co-expressing Mycobacterium neoaurum 3-ketosteroid 9α-hydroxylase and B. subtilis glucose 1-dehydrogenase with NADH regenerationSDS-PAGE analysis of KSH and GDH co-expressed in B. subtilis. Lanes: M protein marker (Takara Biotechnology Co., Ltd., Dalian, China); 1 cell-free extract of B. subtilis 168; 2 cell-free extract of B. subtilis 168/pMA5-ksh–gdh Back to article page