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Fig. 2 | SpringerPlus

Fig. 2

From: Efficient 9α-hydroxy-4-androstene-3,17-dione production by engineered Bacillus subtilis co-expressing Mycobacterium neoaurum 3-ketosteroid 9α-hydroxylase and B. subtilis glucose 1-dehydrogenase with NADH regeneration

Fig. 2

SDS-PAGE analysis of cell-free extract and purified KshA and KshB. Lanes: M protein marker (Takara Biotechnology Co., Ltd., Dalian, China); 1 cell-free extract of B. subtilis 168; 2 cell-free extract of B. subtilis 168/pMA5-kshA; 3 cell-free extract of B. subtilis 168/pMA5-kshB; 4 cell-free extract of B. subtilis 168/pMA5-ksh; 5 purified KshA (45.1 kDa); 6 purified KshB (37.8 kDa); 7 copurified KshA and KshB

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