Table 3 Multiplex PCR based fingerprints of vancomycin-resistance and multiple-virulence-markers in E. faecalis isolates from river Gomti
E. faecalis isolates | Target genes | |||||
|---|---|---|---|---|---|---|
Housekeeping gene | Antimicrobial resistance and Virulence genes | |||||
sodA (E. faecalis) | vanB | gelE | ace | efaA | Esp | |
E. faecalis ATCC 51299 (ATCC, USA) (positive control) | + | + | +P | + | + | + |
Site #1 | ||||||
2E | + | − | + | − | + | + |
AE | + | − | +P | − | + | + |
BE | + | − | +P | + | + | + |
FE | + | + | + | − | + | + |
GE | + | + | +P | − | + | + |
Site #2 | ||||||
4E | + | + | +P | + | + | + |
HE | + | + | +P | − | + | + |
CE | + | + | +P | + | + | + |
G2A | + | − | +P | − | + | + |
G2B | + | − | + | + | + | + |
Site #3 | ||||||
9E | + | − | +P | − | + | + |
IE | + | − | + | − | + | + |
16E | + | − | +P | + | + | + |
G3A | + | − | +P | + | + | + |
G3B | + | − | +P | + | + | + |
G3C | + | − | + | − | + | + |
G3D | + | − | + | − | + | + |
Site #4 | ||||||
11E | + | − | +P | + | + | + |
17E | + | − | + | + | + | + |
18E | + | + | +P | + | + | + |
19E | + | − | +P | − | + | + |
20E | + | + | +P | − | − | + |
G4A | + | − | + | + | + | + |
G4B | + | − | + | + | + | + |
Site #5 | ||||||
15E | + | − | +P | − | + | + |
21E | + | − | + | + | + | + |
22E | + | + | +P | − | + | + |
23E | + | + | +P | + | + | + |
24E | + | − | + | + | + | + |
G5A | + | − | +P | + | + | + |
G5B | + | − | +P | + | + | + |
G6A | + | − | + | + | + | + |
G6B | + | − | +P | + | + | + |