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Table 2 Multiplex PCR primers for vancomycin-resistance and virulence-markers detection in river water isolates of E. faecalis

From: Multiplex PCR based genotypic characterization of pathogenic vancomycin resistant Enterococcus faecalis recovered from an Indian river along a city landscape

Multiplexed/singleplexed genes Oligomer sequence
(5′ → 3′)
Oligo-mer conc.a (µM) Product size
(bp)
Reference
sodA
(E.faecalis)
F: 5′-ACTTATGTGACTAACTTAACC-3′
R: 5′-TAATGGTGAATCTTGGTTTGG-3′
0.15 360 Jackson et al. (2004)
vanB F: 5′-CAAAGCTCCGCAGCTTGCATG-3′
R: 5′-TGCATCCAAGCACCCGATATAC-3′
0.20 484 Dahl et al. (1999)
gelE F: 5′-ACCCCGTATCATTGGTTT-3′
R: 5′-ACGCATTGCTTTTCCATC-3′
0.25 419 Eaton and Gasson (2001)
ace b F: 5′-GGAATGACCGAGAACGATGGC-3′
R: 5′-GCTTGATGTTGGCCTGCTTCCG-3′
0.29 616 Creti et al. (2004)
efaA b F: 5′-GCCAATTGGGACAGACCCTC-3′
R: 5′-CGCCTTCTGTTCCTTCTTTGGC-3′
0.30 688 Creti et al. (2004)
esp c F: 5′-TTGCTAATGCTAGTCCACGACC-3′
R: 5′-GCGTCAACACTTGCATTGCCGAA-3′
0.15 933 Eaton and Gasson (2001)
  1. aOligomer concentration optimized for this study
  2. bMultiplexed genes include either ace or efaA gene with sodA, vanB and gelE genes
  3. cSingleplexed gene