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Fig. 1

From: Expression of a mitochondrial gene orfH79 from CMS-Honglian rice inhibits Escherichia coli growth via deficient oxygen consumption

Fig. 1

Western blot analysis of ORFH79 in whole E. coli extracts and fractions. a Detection of ORFH79 in whole E. coli extracts. Total protein was extracts from pET-28a or pET-28a-orfH79 transformants induced with 1 mM IPTG, and subjected to PAGE in a 15 % acylamide gel. Western blot was preformed to detect the production of ORFH79 protein. Signal were obtained in the protein fraction from pET-28a-orfH79 transformants. No signal appeared in the protein fraction from pET-28a transformants. b Intracellular localization of ORFH79 in E. coli. Crude membrane versus cytoplasmic cell fraction were prepared from pET-28a-orfH79 transformants by series of centrifugation and subjected to PAGE in a 15 % acylamide gel. Western blot was preformed to detect Intracellular localization of ORFH79 in E. coli. Signal were obtained in the crude membrane fraction. M marker; Cyto Cytoplasmic fraction of pET-28a-orfH79 transformants; Mem Crude membrane fraction of pET-28a-orfH79 transformants

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