Table 1 Primers for polymerase chain reaction
cDNA | Primers | Reaction condition |
|---|---|---|
β-actin | 5′-GTGCTATGTTGCTCTAGACTTCG-3′ | 94 °C, 30 s; 55 °C, 30 s; 72 °C, 1 min (30) |
5′-ATGCCACAGGATTCCATACC-3′ | ||
α-SMA | 5′-GGGAGTAATGGTTGGAATGG-3′ | 94 °C, 30 s; 55 °C, 30 s; 72 °C, 1 min (30) |
5′-GGTGATGATGCCGTGTTCTA-3′ | ||
Col-1 | 5′-TGACTGGAAGAGCGGAGAGT-3′ | 94 °C, 30 s; 52 °C, 30 s; 72 °C, 1 min (30) |
5′-CGGCTGAGTAGGGAACACAC-3′ | ||
Col-III | 5′-AGGTTCTCCTGGTGCTGCT-3′ | 94 °C, 30 s; 55 °C, 30 s; 72 °C, 1 min (30) |
5′-ATGCCCACTTGTTCCATCTT-3′ |