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Table 1 Primers for polymerase chain reaction

From: The protective effect of hydrogen sulfide on systemic sclerosis associated skin and lung fibrosis in mice model

cDNA Primers Reaction condition
β-actin 5′-GTGCTATGTTGCTCTAGACTTCG-3′ 94 °C, 30 s; 55 °C, 30 s; 72 °C, 1 min (30)
5′-ATGCCACAGGATTCCATACC-3′
α-SMA 5′-GGGAGTAATGGTTGGAATGG-3′ 94 °C, 30 s; 55 °C, 30 s; 72 °C, 1 min (30)
5′-GGTGATGATGCCGTGTTCTA-3′
Col-1 5′-TGACTGGAAGAGCGGAGAGT-3′ 94 °C, 30 s; 52 °C, 30 s; 72 °C, 1 min (30)
5′-CGGCTGAGTAGGGAACACAC-3′
Col-III 5′-AGGTTCTCCTGGTGCTGCT-3′ 94 °C, 30 s; 55 °C, 30 s; 72 °C, 1 min (30)
5′-ATGCCCACTTGTTCCATCTT-3′
  1. α-SMA α-smooth muscle actin, Col-1 collagen-I, Col-III collagen-III