Table 2 The primers used in this study
Primers | Type | Sequence (5′–3′) | Length (bp) |
|---|---|---|---|
luxR-F3 | F3 | AGCAAAAAGACCCCGCAC | 18 |
luxR-B3 | B3 | CGGTTTCGTTCTCGGTGTT | 20 |
luxR-FIP | F1c F2 | GGCGCGCAAATACATCCAGAGAA-GAATTC- GACTCTCGCCCAAAAAACGT (EcoRI) | 49 |
luxR-BIP | B1c B2 | GTGGGATTGGTCGTGGTGGT-TTTT- ATACCGTGGCGACCGATAC | 43 |
metalloprotease-F3 | F3 | TCAGCAAACCTATTTGGGCC | 20 |
metalloprotease-B3 | B3 | GTCTTCACGTGTTGGGAAGT | 19 |
metalloprotease-FIP | F1c F2 | CCATGCACATTGCTCAACCCTG-TTTT- CCTGTGTTTGATACCGCCG | 45 |
metalloprotease-BIP | B1c B2 | GATCAGCAACAAGCCATCGCC-GGATCC- TACGATTGGCATGCTTCGC (BamHI) | 46 |
ompA-F3 | F3 | AATTACGCGGAAAGAAACC | 19 |
ompA-B3 | B3 | GAGGCTTATTTCAATAACATGC | 22 |
ompA-FIP | F1c F2 | AGCGGTAGGTTACTCATCCTCTAA-TTTT- GGATCGCTTCTCATCTTCA | 47 |
ompA-BIP | B1c B2 | TTACCCACCCGTAGTGTTCG-CTGCAG- CAATCGAGAACTCGTGCC (PstI) | 44 |
ToxR-F3 | F3 | TTTGCCGCTCAGCTAACC | 18 |
ToxR-B3 | B3 | CCCAACCCCAAGTTGAGTT | 19 |
ToxR-FIP | F1c F2 | AAGCGTAACCATGCCGCCAC-GATATC- TACGCGTGGTGTCTATAGCA (EcoRV) | 46 |
ToxR-BIP | B1c B2 | AGTTCAGCCATGAAGTTGGGCA-TTTT- TGGTTTATGAATTGCCCCCT | 46 |