Fig. 4
Sensitivities of the quadruplex LAMP and conventional PCR methods. Lanes 1–9 the quadruplex LAMP (e–h) and general PCR (a–d) were carried out using DNA extracted from different concentrations of V. scophthalmi (a, e), V. vulnificus (b, f), V. parahaemolyticus (c, g) and V. ichthyoenteri (d, h) as template. The bacteria concentration from lanes 1–8 were 8 × 106, 8 × 105, 8 × 104, 8 × 103, 8 × 102, 8 × 101, 8 × 100, and 8 × 10−1 CFU per reaction, respectively. M Marker. All products were electrophoresed on a 2 % agarose gel