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Fig. 1 | SpringerPlus

Fig. 1

From: Development of a quadruplex loop-mediated isothermal amplification assay for field detection of four Vibrio species associated with fish disease

Fig. 1

The optimization of the LAMP reaction temperature. LAMP reaction temperature of V. scophthalmi (a), V. vulnificus (b), V. parahaemolyticus (c) and V. ichthyoenteri (d) was set at 58–65 °C with 1 °C intervals, respectively. Lanes 2, 4, 6, 8, 10, 12, 14, and 16 were the amplification products, lanes 1, 3, 5, 7, 9, 11, 13, and 15 were negative control (the application templates used was ddH2O). M Marker. All products were electrophoresed on a 2 % agarose gel

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