Table 3 Molecular characterization (partial ITS sequencing) and mating types of Tuber spp. mycelial cultures
Mycelial culture | Sequence length (bp) | Accession no | Closest BLASTn (accession no) | Identity | Mating type |
|---|---|---|---|---|---|
BTR3 | 603 | KM659869 | T. melanosporum (AF132501) | 100 | MAT 1-2-1 |
Mel14 | nd | nd | nd | nd | MAT 1-1-1 |
Mel18 | 591 | KM659866 | T. melanosporum (GU979083) | 100 | MAT 1-2-1 |
Mel28 | 550 | KM659874 | T. melanosporum (GU979083) | 100 | MAT 1-2-1 |
Mel2VDA3 | 604 | KM659868 | T. melanosporum (GU979083) | 100 | MAT 1-2-1 |
Mel3VDA4 | 598 | KM659867 | T. melanosporum (GU979083) | 100 | MAT 1-1-1 |
MelBal1 | 604 | KM659870 | T. melanosporum (AF300826) | 99 | MAT 1-2-1 |
MelBal3 | 571 | KM659873 | T. melanosporum (GU810153) | 99 | MAT 1-2-1 |
MelC89 | 609 | KM659871 | T. melanosporum (GU979083) | 99 | MAT 1-1-1 |
MelCR2-00 | 610 | KM659872 | T. melanosporum (GU810153) | 99 | MAT 1-2-1 |
MelBaud1 | nd | nd | nd | nd | nd |
TBRS | 880 | KM659875 | T. brumale (JF926118) | 99 | MAT 1-2-1 |