Fig. 1From: Binding interaction of a gamma-aminobutyric acid derivative with serum albumin: an insight by fluorescence and molecular modeling analysisFluorescence quenching of BSA and HSA with the GABA derivative. Serum albumin concentration was kept constant at 0.5 μM (in 20 mM Tris–HCl buffer of pH 7.0) and the ligand concentration was varied from 0 to 5.5 μM. a Spectral changes showing the quenching of intrinsic fluorescence emission of BSA as a function of ligand concentration. b Quenching of intrinsic fluorescence of HSA in presence of ligand. These two spectra were recorded at room temperatureBack to article page