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Fig. 9 | SpringerPlus

Fig. 9

From: Preliminary characterization of a novel β-agarase from Thalassospira profundimonas

Fig. 9

LC-MS/MS spectrum of the enzymatic products of agarose resulting from agarase-fst. Samples were resuspended with 5 µl 0.1 % (w/v) TFA followed by mixing (1:1, v/v) with a matrix consisting of a saturated solution of α-cyano-4-hydroxy-trans-cinnamic acid in 50 % (w/v) ACN, 0.1 % (w/v) TFA. And 1 μl mixture was spotted on a stainless steel sample target plate. The molecular mass distribution was determined using MS Agilent LC1290-Triple-Q 6410 (Agilent Technologies, USA). Ionization was achieved by atmospheric pressure electrospray ionization in the positive mode with an ESI capillary voltage of 4 kV. The mass spectra were recorded in the range of m/z 50 and1500

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