Molecular cloning, expression analysis and subcellular localization of four DELLA genes from hybrid poplar

Liu, Sian; Xuan, Lei; Xu, Li-An; Huang, Minren; Xu, Meng

doi:10.1186/s40064-016-2728-x

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Table 1 Primer sequences of the four poplar DELLA genes

From: Molecular cloning, expression analysis and subcellular localization of four DELLA genes from hybrid poplar

Primer_ID	Forward PCR primer (5′–3′)	Reverse PCR primer (5′–3′)
PeRGA1_3OUTER	CATCAAGAAACCATTGGTGGTGCT	TACCGTCGTTCCACTAGTGATTT
PeRGA1_3INNER	CAAAGCTGAATCTTCTTCTTCGTCAAT	CGCGGATCCTCCACTAGTGATTTCACTATAGG
PeRGA1_5OUTER	CATGGCTACATGCTGACAGCCTA	CCCAACTAGGGTGAGCTTCATTCG
PeRGA1_5INNER	CGCGGATCCACAGCCTACTGATGATCAGTCGATG	ATCGAATTCTTGTTGAGGATAGGCAGC
PeRGA1_ORF	ATGAAGAGAGATCATCAAGA	TCATTGACTCGGTAGCTCGA
PeRGA1_sRT-PCR	AAGCTTGTTTGACTCACTC	ACGTTTCATGTCGCT
PeRGA1_qRT-PCR	GTTGGGTTCAAAGCATG	GGGAATTGCTCTGAGAT
PeRGA2_3OUTER	CACTATAACCCTTCAGATCTCT	TACCGTCGTTCCACTAGTGATTT
PeRGA2_3INNER	TCAACAATCTACCTTCTACTGATCTTGA	CGCGGATCCTCCACTAGTGATTTCACTATAGG
PeRGA2_5OUTER	CATGGCTACATGCTGACAGCCTA	ATTGCATTCCCTGTTTTAAACC
PeRGA2_5INNER	CGCGGATCCACAGCCTACTGATGATCAGTCGATG	CTCGACTTGCATTCGCGAAAGCTTCAAG
PeRGA2_ORF	ATGAAGAGAGATCATCAAGAA	TCATTGTTGTGAATCACCAG
PeRGA2_sRT-PCR	ACTGATCTTGATTCATCTA	TGCCGGCGCAATATTAGACC
PeRGA2_qRT-PCR	CTTAGATTTCCCCAGTAA	CTAGAAAAACCGGACCGT
PeGAI1_3OUTER	CTTACTAGCACCGGTACTATGAC	TACCGTCGTTCCACTAGTGATTT
PeGAI1_3INNER	TGGATGAACTTTTAGCTGTTTTGGGTTA	CGCGGATCCTCCACTAGTGATTTCACTATAGG
PeGAI1_5OUTER	CATGGCTACATGCTGACAGCCTA	ACTCACTGATCCCTCCAACGAG
PeGAI1_5INNER	CGCGGATCCACAGCCTACTGATGATCAGTCGATG	CTCCGGTTTCATTTGTTTCACAACTGAT
PeGAI1_ORF	ATGAAAAGAGAACACTCAA	TTAAGCAGCACCGCCTACTGG
PeGAI1_sRT-PCR	GAGATTGTTACTGTCGTTG	CACTGAGTCAGGGTC
PeGAI1_qRT-PCR	GTCAAACAAATCGGCTT	AATATCTGAGAGAGAGT
PeGAI2_3OUTER	TAAGATCGTCAGACATGGCTGA	TACCGTCGTTCCACTAGTGATTT
PeGAI2_3INNER	CACGCGCAAGAAGATGGTCTTTCCCAC	CGCGGATCCTCCACTAGTGATTTCACTATAGG
PeGAI2_5OUTER	CATGGCTACATGCTGACAGCCTA	CTTCCCCAAGTACACCTCTGAC
PeGAI2_5INNER	CGCGGATCCACAGCCTACTGATGATCAGTCGATG	ATTATGGTTCGCTTCTTGCTCAACAACA
PeGAI2_ORF	ATGAAAAGAGAACACCCA	CTAAGCAGCACCAACTACCG
PeGAI2_sRT-PCR	GCATCTGATTCTGTCCATT	TGGTCGGAGAAATCGATAG
PeGAI2_qRT-PCR	GACCCCTCTGCTGATTCTT	TATAGGTCTGTTTTTAA
EF1α_qRT-PCR	GGCAAGGAGAAGGTACACAT	CAATCACACGCTTGTCAATA
18S_sRT-PCR	TCAACTTTCGATGGTAGGATAGTG	CCGTGTCAGGATTGGGTAATTT

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