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Table 3 Densitometric analysis of DNA in plasmid nicking assay of extract/fractions of M. exotica leaves

From: Delineation of attenuation of oxidative stress and mutagenic stress by Murraya exotica L. leaves

Form of DNA Amount of DNA (%)a
1 2 3 4 5 6 7 8
Ethanol extract
 Form I 69.8 30.7 52.1 51.8 49.8 43.8 41.5
 Form II 30.2 71.4 54.9 40.2 40.5 42.5 50.0 53.0
 Form III 28.6 14.4 7.8 7.7 7.7 6.2 5.5
Hexane fraction
 Form I 81.6 17.7 43.2 41.2 42.0
 Form II 18.4 72.6 66.5 46.4 53.3 43.0 79.6 73.4
 Form III 27.4 15.9 10.4 8.9 15.0 20.1 26.6
Chloroform fraction
 Form I 68.3
 Form II 31.7 63.15 48.1 49.1 55 55.4 54.2 55.7
 Form III 36.85 51.9 50.9 45 44.6 45.8 44.3
Ethyl acetate fraction
 Form I 79.1 39.1 58.2 55.9 55.2 52.9 40.3
 Form II 20.9 76.3 45.8 35.4 35.2 36.0 38.1 49.7
 Form III 23.7 15.1 6.4 8.9 8.8 9.0 10.0
n-butanol fraction
 Form I 69
 Form II 31 51.9 45.2 47.3 48.6 51 52.6 55.3
 Form III 48.1 54.8 52.7 51.4 49 47.4 44.7
Aqueous fraction
 Form I 72.4
 Form II 27.6 65.1 50.4 51.1 51.6 52.8 53.8 56.3
 Form III 34.9 49.6 48.9 48.4 47.2 46.2 43.7
  1. aLane 1: pBR322 DNA + distilled water; lane 2: pBR322 DNA + distilled water + Fenton Reagent (FR); lane 3: pBR322 DNA + FR + Rutin; lane 4–8: pBR322 DNA + FR + Extract/fraction (1000, 800, 600, 400 and 200 µg/ml respectively)