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Table 7 Growth inhibitory efficiency of antimicrobial substances against bacterial contaminants in sterilized creamy filling containing about 5 log CFU/g of each target strain and various food preservatives of PP-KLD, H-3 and glycine

From: Characterization of antimicrobial substance from Lactobacillus salivarius KL-D4 and its application as biopreservative for creamy filling

Target strain Treatment
% (w/w)a
Growth inhibitory efficiency (%)b
0 h 72 h
Enterococcus fecalis KUB-E5 1 %PP-KLD 22.76 ± 0.82b 52.27 ± 2.09b
  5 %PP-KLD 39.30 ± 3.08c 100.00 ± 0.00c
  10 %PP-KLD 26.46 ± 0.40b 100.00 ± 0.00c
  1 %H-3 0.39 ± 0.16a 1.75 ± 0.27a
  2 %Glycine 1.27 ± 0.32a 1.87 ± 0.45a
Enterococcus fecalis KUB-E7 1 %PP-KLD 15.40 ± 3.14b 11.51 ± 5.34b
  5 %PP-KLD 17.83 ± 2.43b 30.69 ± 0.28c
  10 %PP-KLD 19.96 ± 0.94b 60.12 ± 0.4d
  1 %PP-H-3 1.88 ± 0.08a 0.90 ± 0.13a
  2 %PP-Glycine 3.61 ± 0.39a 3.89 ± 0.15a
Staphylococcus epidermidis KUB-E6 1 %KLD 1.83 ± 1.02b 0.00 ± 0.00a
  5 %KLD 0.46 ± 2.36a 0.00 ± 0.00a
  10 %KLD 1.80 ± 2.55b 0.00 ± 0.00a
  1 %H-3 3.24 ± 0.38c 24.58 ± 1.11b
  2 %Glycine 0.00 ± 0.00a 22.37 ± 1.14b
Staphylococcus hominis KUB-E8 1 %PP-KLD 1.96 ± 1.95a 22.91 ± 0.23a
  5 %PP-KLD 3.85 ± 2.66a 28.10 ± 1.38c
  10 %PP-KLD 3.37 ± 0.17a 25.80 ± 1.85c
  1 %H-3 2.58 ± 0.18a 23.05 ± 0.28ab
  2 %Glycine 10.54 ± 0.27b 25.69 ± 0.28bc
Bacillus cereus JCM2152 1 %PP-KLD 2.02 ± 1.43a 4.47 ± 2.43a
  5 %PP-KLD 6.18 ± 0.41b 50.39 ± 1.58b
  10 %PP-KLD 10.14 ± 0.29c 66.94 ± 0.88c
  1 %H-3 3.28 ± 0.19a 44.29 ± 0.29b
  2 %Glycine 8.85 ± 0.22c 6.97 ± 0.17a
Pseudomonas stutzeri KUB-E1 1 %PP-KLD 3.92 ± 5.93a 100.00 ± 0.00c
  5 %PP-KLD 100.00 ± 0.00c 100.00 ± 0.00c
  10 %PP-KLD 100.00 ± 0.00c 100.00 ± 0.00c
  1 %H-3 11.35 ± 1.15b 12.53 ± 0.58a
  2 %Glycine 14.96 ± 0.31b 64.07 ± 2.34b
Stenotrophomonas sp. KUB-E2 1 %PP-KLD 0.27 ± 0.16a 5.25 ± 2.56a
  5 %PP-KLD 0.00 ± 0.00a 100.00 ± 0.00d
  10 %PP-KLD 1.50 ± 0.40b 100.00 ± 0.00d
  1 %H-3 5.82 ± 0.05c 9.04 ± 0.36b
  2 %Glycine 6.24 ± 0.54c 44.84 ± 1.44c
  1. All growth efficiency obtained are a mean of two replications ± SD (SD); a–d, means in the same column of each target strain with different lower case letters are significantly different (P < 0.05)
  2. aFive treatments of antimicrobial substance PP-KLD at the concentrations of 1, 5 and 10 %, 1 % H3 and 2 % glycine tested
  3. bGrowth inhibitory efficiency defined as percentage of survival cells after antimicrobial substance treatment compared to the control mentioned elsewhere