Characterization of antimicrobial substance from Lactobacillus salivarius KL-D4 and its application as biopreservative for creamy filling

Therdtatha, Phatthanaphong; Tandumrongpong, Chanabhorn; Pilasombut, Komkhae; Matsusaki, Hiromi; Keawsompong, Suttipun; Nitisinprasert, Sunee

doi:10.1186/s40064-016-2693-4

SpringerPlus

Table 7 Growth inhibitory efficiency of antimicrobial substances against bacterial contaminants in sterilized creamy filling containing about 5 log CFU/g of each target strain and various food preservatives of PP-KLD, H-3 and glycine

From: Characterization of antimicrobial substance from Lactobacillus salivarius KL-D4 and its application as biopreservative for creamy filling

Target strain	Treatment % (w/w)^a	Growth inhibitory efficiency (%)^b
Target strain	Treatment % (w/w)^a	0 h	72 h
Enterococcus fecalis KUB-E5	1 %PP-KLD	22.76 ± 0.82b	52.27 ± 2.09b
	5 %PP-KLD	39.30 ± 3.08c	100.00 ± 0.00c
	10 %PP-KLD	26.46 ± 0.40b	100.00 ± 0.00c
	1 %H-3	0.39 ± 0.16a	1.75 ± 0.27a
	2 %Glycine	1.27 ± 0.32a	1.87 ± 0.45a
Enterococcus fecalis KUB-E7	1 %PP-KLD	15.40 ± 3.14b	11.51 ± 5.34b
	5 %PP-KLD	17.83 ± 2.43b	30.69 ± 0.28c
	10 %PP-KLD	19.96 ± 0.94b	60.12 ± 0.4d
	1 %PP-H-3	1.88 ± 0.08a	0.90 ± 0.13a
	2 %PP-Glycine	3.61 ± 0.39a	3.89 ± 0.15a
Staphylococcus epidermidis KUB-E6	1 %KLD	1.83 ± 1.02b	0.00 ± 0.00a
	5 %KLD	0.46 ± 2.36a	0.00 ± 0.00a
	10 %KLD	1.80 ± 2.55b	0.00 ± 0.00a
	1 %H-3	3.24 ± 0.38c	24.58 ± 1.11b
	2 %Glycine	0.00 ± 0.00a	22.37 ± 1.14b
Staphylococcus hominis KUB-E8	1 %PP-KLD	1.96 ± 1.95a	22.91 ± 0.23a
	5 %PP-KLD	3.85 ± 2.66a	28.10 ± 1.38c
	10 %PP-KLD	3.37 ± 0.17a	25.80 ± 1.85c
	1 %H-3	2.58 ± 0.18a	23.05 ± 0.28ab
	2 %Glycine	10.54 ± 0.27b	25.69 ± 0.28bc
Bacillus cereus JCM2152	1 %PP-KLD	2.02 ± 1.43a	4.47 ± 2.43a
	5 %PP-KLD	6.18 ± 0.41b	50.39 ± 1.58b
	10 %PP-KLD	10.14 ± 0.29c	66.94 ± 0.88c
	1 %H-3	3.28 ± 0.19a	44.29 ± 0.29b
	2 %Glycine	8.85 ± 0.22c	6.97 ± 0.17a
Pseudomonas stutzeri KUB-E1	1 %PP-KLD	3.92 ± 5.93a	100.00 ± 0.00c
	5 %PP-KLD	100.00 ± 0.00c	100.00 ± 0.00c
	10 %PP-KLD	100.00 ± 0.00c	100.00 ± 0.00c
	1 %H-3	11.35 ± 1.15b	12.53 ± 0.58a
	2 %Glycine	14.96 ± 0.31b	64.07 ± 2.34b
Stenotrophomonas sp. KUB-E2	1 %PP-KLD	0.27 ± 0.16a	5.25 ± 2.56a
	5 %PP-KLD	0.00 ± 0.00a	100.00 ± 0.00d
	10 %PP-KLD	1.50 ± 0.40b	100.00 ± 0.00d
	1 %H-3	5.82 ± 0.05c	9.04 ± 0.36b
	2 %Glycine	6.24 ± 0.54c	44.84 ± 1.44c

All growth efficiency obtained are a mean of two replications ± SD (SD); a–d, means in the same column of each target strain with different lower case letters are significantly different (P < 0.05)
^aFive treatments of antimicrobial substance PP-KLD at the concentrations of 1, 5 and 10 %, 1 % H3 and 2 % glycine tested
^bGrowth inhibitory efficiency defined as percentage of survival cells after antimicrobial substance treatment compared to the control mentioned elsewhere

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