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Table 6 Purification table of salivaricin KLD

From: Characterization of antimicrobial substance from Lactobacillus salivarius KL-D4 and its application as biopreservative for creamy filling

Purification stage

Vol. (ml)

Activity (AU/ml)

Protein concentration (mg/ml)

Total protein (mg)

Total activity (AU)

Specific activity (AU/mg)

Purification (folds)

Yield (%)

Cell free supernatant

1000

25,600

18.600

18,600.000

2.56 × 107

1.38 × 103

1

100

(NH4)2SO4 precipitate

20

102,400

2.150

43.000

2.05 × 106

4.77 × 104

34.57

8.01

Cation exchange

20

12,800

0.224

4.480

2.56 × 105

5.71 × 104

41.38

1.00

RP-HPLC

1

3200

0.057

0.057

3.20 × 103

5.61 × 104

40.68

0.01

  1. All % yield values are expressed in terms of activity units in the cell free supernatant taken as 100 % while purification values are in the terms of specific activities in the cell free supernatant taken as 1