Purification stage | Vol. (ml) | Activity (AU/ml) | Protein concentration (mg/ml) | Total protein (mg) | Total activity (AU) | Specific activity (AU/mg) | Purification (folds) | Yield (%) |
---|---|---|---|---|---|---|---|---|
Cell free supernatant | 1000 | 25,600 | 18.600 | 18,600.000 | 2.56 × 107 | 1.38 × 103 | 1 | 100 |
(NH4)2SO4 precipitate | 20 | 102,400 | 2.150 | 43.000 | 2.05 × 106 | 4.77 × 104 | 34.57 | 8.01 |
Cation exchange | 20 | 12,800 | 0.224 | 4.480 | 2.56 × 105 | 5.71 × 104 | 41.38 | 1.00 |
RP-HPLC | 1 | 3200 | 0.057 | 0.057 | 3.20 × 103 | 5.61 × 104 | 40.68 | 0.01 |