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Table 6 Purification table of salivaricin KLD

From: Characterization of antimicrobial substance from Lactobacillus salivarius KL-D4 and its application as biopreservative for creamy filling

Purification stage Vol. (ml) Activity (AU/ml) Protein concentration (mg/ml) Total protein (mg) Total activity (AU) Specific activity (AU/mg) Purification (folds) Yield (%)
Cell free supernatant 1000 25,600 18.600 18,600.000 2.56 × 107 1.38 × 103 1 100
(NH4)2SO4 precipitate 20 102,400 2.150 43.000 2.05 × 106 4.77 × 104 34.57 8.01
Cation exchange 20 12,800 0.224 4.480 2.56 × 105 5.71 × 104 41.38 1.00
RP-HPLC 1 3200 0.057 0.057 3.20 × 103 5.61 × 104 40.68 0.01
  1. All % yield values are expressed in terms of activity units in the cell free supernatant taken as 100 % while purification values are in the terms of specific activities in the cell free supernatant taken as 1