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Table 3 Inhibitory activities against Lactobacillus sakei subsp. sakei jcm1157T of cell free supernatant under various conditions

From: Characterization of antimicrobial substance from Lactobacillus salivarius KL-D4 and its application as biopreservative for creamy filling

Treatment

Activity (AU/ml)

Enzymatic stability

 

 Control

12,800

 Protenase K pH 8.0

0

 Pepsin pH 3.0

0

 Trypsin pH 8.5

0

 α-chymotrypsin pH 8.5

0

 Actinase E pH 7.0

0

 α-amylase pH 7.0

12,800

 Lipase pH 7.0

12,800

Heat stability

 

 100 °C for 5 min, pH 5.5

25,600

 100 °C for 30 min, pH 5.5

25,600

 121 °C for 15 min, pH 5.5

25,600

pH stability

 

 pH 3.0

12,800

 pH 4.0

12,800

 pH 5.0

12,800

 pH 5.5

25,600

 pH 6.0

25,600

 pH 7.0

25,600

 pH 8.0

12,800

 pH 9.0

12,800

 pH 10.0

12,800