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Table 2 List of primers and nucleotide sequences

From: Characterization of antimicrobial substance from Lactobacillus salivarius KL-D4 and its application as biopreservative for creamy filling

Primer set Sequence 5′–3′ Purpose
SalAα-f1 5′-ATGAAGGAATTTACAGTATTGACAGAATGT-3′ To amplify kld1
SalAα-r1 5′-TTATAAACAAGTAAGCGCTCCGCCTACCAT-3′  
D4-f1 5′-GCTGGTATTGTAGGCGGAGCAAACTTAGGA-3′ To amplify kld2,3,4
Oligo105 5′-CYTCDATNGCRTTRTC-3′*  
D4-f1 5′-GCTGGTATTGTAGGCGGAGCAAACTTAGGA-3′ To amplify kld5
D4-r1 5′-CTACACAATTAGGATAACGTTTCCCACCGT-3  
D4-f2 5′-GATTAAGACATTCAAATCAAGATATTTGTA-3′ To confirm kld locus
D4-r2 5′-ATGTAACGACTATAAATACAATATTTTGTT-3′  
T7 promoter 5′-TAATACGACTCACTATAGGG-3′ Colony PCR for pT7Blue-T vector
U-19mer 5′-GTTTTCCCAGTCACGACGT-3′  
M13RV 5′-CAGGAAACAGCTATGAC-3′ Colony PCR for pMD20-T vector
M13M4 5′-GTTTTCCCAGTCACGAC-3′  
  1. * D = A, G, T; R = A, G; Y = C, T