Primer set | Sequence 5′–3′ | Purpose |
---|---|---|
SalAα-f1 | 5′-ATGAAGGAATTTACAGTATTGACAGAATGT-3′ | To amplify kld1 |
SalAα-r1 | 5′-TTATAAACAAGTAAGCGCTCCGCCTACCAT-3′ |  |
D4-f1 | 5′-GCTGGTATTGTAGGCGGAGCAAACTTAGGA-3′ | To amplify kld2,3,4 |
Oligo105 | 5′-CYTCDATNGCRTTRTC-3′* |  |
D4-f1 | 5′-GCTGGTATTGTAGGCGGAGCAAACTTAGGA-3′ | To amplify kld5 |
D4-r1 | 5′-CTACACAATTAGGATAACGTTTCCCACCGT-3 |  |
D4-f2 | 5′-GATTAAGACATTCAAATCAAGATATTTGTA-3′ | To confirm kld locus |
D4-r2 | 5′-ATGTAACGACTATAAATACAATATTTTGTT-3′ |  |
T7 promoter | 5′-TAATACGACTCACTATAGGG-3′ | Colony PCR for pT7Blue-T vector |
U-19mer | 5′-GTTTTCCCAGTCACGACGT-3′ |  |
M13RV | 5′-CAGGAAACAGCTATGAC-3′ | Colony PCR for pMD20-T vector |
M13M4 | 5′-GTTTTCCCAGTCACGAC-3′ |  |