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Table 2 List of primers and nucleotide sequences

From: Characterization of antimicrobial substance from Lactobacillus salivarius KL-D4 and its application as biopreservative for creamy filling

Primer set

Sequence 5′–3′

Purpose

SalAα-f1

5′-ATGAAGGAATTTACAGTATTGACAGAATGT-3′

To amplify kld1

SalAα-r1

5′-TTATAAACAAGTAAGCGCTCCGCCTACCAT-3′

 

D4-f1

5′-GCTGGTATTGTAGGCGGAGCAAACTTAGGA-3′

To amplify kld2,3,4

Oligo105

5′-CYTCDATNGCRTTRTC-3′*

 

D4-f1

5′-GCTGGTATTGTAGGCGGAGCAAACTTAGGA-3′

To amplify kld5

D4-r1

5′-CTACACAATTAGGATAACGTTTCCCACCGT-3

 

D4-f2

5′-GATTAAGACATTCAAATCAAGATATTTGTA-3′

To confirm kld locus

D4-r2

5′-ATGTAACGACTATAAATACAATATTTTGTT-3′

 

T7 promoter

5′-TAATACGACTCACTATAGGG-3′

Colony PCR for pT7Blue-T vector

U-19mer

5′-GTTTTCCCAGTCACGACGT-3′

 

M13RV

5′-CAGGAAACAGCTATGAC-3′

Colony PCR for pMD20-T vector

M13M4

5′-GTTTTCCCAGTCACGAC-3′

 
  1. * D = A, G, T; R = A, G; Y = C, T