Fig. 4
Characterization of purified salivaricin KLD. Silver stained Tris–Tricine SDS-PAGE containing 15.0 % polyacrylamide gel (a), Protein with antibacterial activity visualized by overlaying soft MRS agar containing cells of Lb. sakei subsp. sakei JCM 1157T (b); lane 1 Spectra Multicolor Low Range Protein Ladder (Thermo Scientific Pierce, USA); lane 2: purified salivaricin KLD. The arrows indicate a salivaricin KLD fraction and its activity