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Fig. 5 | SpringerPlus

Fig. 5

From: Preparation of a functional fluorescent human Fas ligand extracellular domain derivative using a three-dimensional structure guided site-specific fluorochrome conjugation

Fig. 5

Analysis of the complex formation by high-performance size-exclusion chromatography. The mixtures of FL-5Mal conjugated NFG1CG4-hFasLECD (L) and hFasRECD-Fc (R) in four different amount ratios were examined. a L 5.0 μg and R 19.4 μg, b L 7.5 μg and R 19.4 μg, c L 15 μg and R 19.4 μg, d L 30 μg and R 19.4 μg. Used column, Superdex 200 10/300 GL. Elution buffer, 50 mM Tris-hydrochloride plus 150 mM sodium chloride (pH 7.5). Absorbance at 280 nm (blue) and 495 nm (red) was used for the detection. Flow rate, 0.5 ml/min. The retention time of each peak position is shown

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