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Fig. 4 | SpringerPlus

Fig. 4

From: Preparation of a functional fluorescent human Fas ligand extracellular domain derivative using a three-dimensional structure guided site-specific fluorochrome conjugation

Fig. 4

Spectroscopic analysis of FL-5Mal conjugated NFG1CG4-hFasLECD. The purified peak 2 sample of the FL-5Mal conjugated NFG1CG4-hFasLECD was used. a UV–Vis spectrum (250–600 nm). b Fluorescence emission spectrum (400–700 nm) excited at 495 nm. Insert a fluorescence emission observed in the measurement cuvette

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