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Fig. 2 | SpringerPlus

Fig. 2

From: Preparation of a functional fluorescent human Fas ligand extracellular domain derivative using a three-dimensional structure guided site-specific fluorochrome conjugation

Fig. 2

Conjugation of FL-5Mal to NFG1CG4-hFasLECD. a Experimental procedures. b SDS-PAGE analysis of the conjugation reaction and resolution by the first size-exclusion chromatography. Lanes: M molecular-weight size markers (14.4, 20.1, 30.0, 42.4, 66.3 and 97.4 kDa), 1 TCEP reduced sample before conjugation reaction, 2 after conjugation reaction, 3 after quenching reaction, 4 initial flow-through fraction (2.5 ml) in the first size-exclusion chromatography, 5–9 following fractions (1 ml each). Used column, PD-10. Thick and thin arrows indicate the positions of FL-5Mal conjugated hFasLECD and P. pastoris AOX-1, respectively

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