Table 1 Results obtained for the validation of the HPLC/UV method used in the current study
Tea component | Concentration (µg/mL) | Accuracy (µg/mL) | Precision | LOD (μg/mL) | LOQ (μg/mL) | |
|---|---|---|---|---|---|---|
Within run | Between run | |||||
Gallic acid | 3 | 2.9 ± 0.1 | 3.8 | 4.1 | 0.4 | 1.2 |
4 | 4.1 ± 0.3 | 5.1 | 7.4 | |||
7 | 7.2 ± 0.5 | 3.0 | 6.6 | |||
15 | 15.0 ± 1.0 | 1.9 | 7.0 | |||
Caffeine | 3 | 3.1 ± 0.1 | 2.1 | 4.6 | 0.7 | 2.2 |
4 | 4.0 ± 0.2 | 3.4 | 6.4 | |||
7 | 7.2 ± 0.4 | 0.9 | 5.5 | |||
15 | 15.5 ± 0.9 | 1.6 | 6.0 | |||
Epicatechin | 3 | 3.0 ± 0.1 | 2.3 | 2.0 | 1.3 | 3.8 |
4 | 4.3 ± 0.3 | 3.3 | 6.9 | |||
7 | 6.8 ± 0.4 | 2.8 | 5.5 | |||
15 | 14.5 ± 1.0 | 0.8 | 6.6 | |||
Epigallocatechin gallate | 3 | 3.0 ± 0.1 | 3.6 | 3.9 | 0.7 | 2.2 |
4 | 3.8 ± 0.1 | 6.2 | 2.3 | |||
7 | 6.5 ± 0.4 | 2.7 | 6.7 | |||
15 | 15.4 ± 0.9 | 2.8 | 5.9 | |||